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Ergocalciferol Hplc . A standard stock solution of cholecalciferol (762 ppm) and ergocalciferol (1000 ppm) was made in a glycerin/water solution and diluted 1:20 with 50/50 acetonitrile/water. (ergocalciferol) and vitamin d3 (cholecalciferol) in greenhouse and ordinary apricots were compared together, respectively. HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis from www.sigmaaldrich.com Vitamin d2 and d3 may be used in a wide range of studies to assess their effects on function such as immune function and calcium homeostasis. Calibration curves were found to be linear within the previously mentioned concentration ranges, with a minimum correlation coefficient of 0.9940. Both forms are rarely added to the same product.